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| Barranger, Audrey; Langan, Laura M; Sharma, Vikram; Rance, Graham A; Aminot, Yann; Weston, Nicola J; Akcha, Farida; Moore, Michael N; Arlt, Volker M; Kholbystov, Andrei N; Readman, James W; Jha, Awadhesh N.. |
| This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR).... |
| Tipo: Text |
Palavras-chave: Trojan Horse effect; B[a]P; NC60; Co-exposure; Mussels; DNA damage; Proteomics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00498/60946/64340.pdf |
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| Barranger, Audrey; Langan, Laura M.; Sharma, Vikram; Rance, Graham A.; Aminot, Yann; Weston, Nicola J.; Akcha, Farida; Moore, Michael N.; Arlt, Volker M.; Khlobystov, Andrei N.; Readman, James W.; Jha, Awadhesh N.. |
| This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography–Mass Spectrometry (GC–MS), Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP–MS). Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the interactive effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1... |
| Tipo: Text |
Palavras-chave: Trojan horse effect; B[a]P; NC(60); Co-exposure; Mussels; DNA damage; Proteomics. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00506/61780/65779.pdf |
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| Akcha, Farida. |
| In the aim to develop biomarkers of genotoxicity for the biomonitoring of the marine environment,the formation of DNA adducts and oxidized DNA bases was studied for the sentinel organism, Mytilus sp, following exposure to B[a]P via the trophic supply. DNA adducts and 8-oxo-7,8-dihydro-2'-désoxyguanosine (8-oxodGuo) were respectively measured by 32P post-labeling technique and high performance liquid chromatography coupled to electrochemical detection. The developped model of exposure was validated for the realisation of toxicological studies in Mytilus sp. The bioavailability of B[a]P for the exposed organisms and its biotransformation resulted in variations in most of the measured enzymatic biomarkers of exposure. Such an exposure allowed the expression... |
| Tipo: Text |
Palavras-chave: Moule; B[a]P; Adduits; 8-oxodGuo; Biomarqueurs; Mussel; B[a]P; Adducts; 8-oxodGuo; Biomarkers. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00076/18770/16349.pdf |
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| Silva,Grazyelle Sebrenski da; Fé,Luciana Mara Lopes; Silva,Maria de Nazaré Paula da; Val,Vera Maria Fonseca de Almeida e. |
| Abstract Benzo[a]pyrene (B[a]P) is a petroleum derivative capable of inducing cancer in human and animals. In this work, under laboratory conditions, we analyzed the responses of Colossoma macropomum to B[a]P acute exposure through intraperitoneal injection of four different B[a]P concentrations (4, 8, 16 and 32 μmol/kg) or corn oil (control group). We analyzed expression of the ras oncogene and the Hypoxia-inducible factor-1 alpha (hif-1α) gene using quantitative real-time PCR. Additionally, liver histopathological changes and genotoxic effects were evaluated through the comet assay. Ras oncogene was overexpressed in fish exposed to 4, 8 of 16 μmol/kg B[a]P, showing 4.96, 7.10 and 6.78-fold increases, respectively. Overexpression also occurred in hif-1α... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ras oncogene; Hif-1α; Tambaqui; B[a]P; Genotoxic damage. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300491 |
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